The purpose of this research is to find out the self-efficacy of pre-service mathematics teachers in online learning based on video conferences. This research is a descriptive quantitative study with the population of all pre-service mathematics teachers of Malikussaleh University for the 2020/2021 academic year. The sample was selected using purposive sampling techniques which amounted to 41 pre-service mathematics teachers. The instrument used is a self-efficacy questionnaire containing 25 statements that have been tested and analyzed for validation. The questionnaire was given after 4 meetings were conducted online video conference-based learning. The results showed that through online learning based on video conference, 24.4% of pre-service mathematics teachers have high self-efficacy, 56.1% of pre-service mathematics teachers with moderate self-efficacy, and 19.5% at a low level.